Expression from Diverse Hosts
Microbial & mammalian expression system optimization
We eliminate development bottlenecks by selecting and optimizing the ideal expression system for your specific protein. Our expertise spans microbial (E. coli, yeast) and eukaryotic (mammalian cell lines) hosts, ensuring maximum yield and correct post-translational modifications for functional activity.
Key Capabilities
- E. coli & yeast expression systems
- Mammalian cell line development (CHO, HEK)
- Codon optimization & vector design
- Post-translational modification verification
- Scalable expression protocols
Extensive Purification Expertise
Multi-step chromatographic protocols for therapeutic-grade purity
Achieving therapeutic-grade purity is our specialty. Leveraging advanced chromatographic techniques — including Affinity, Ion Exchange, Hydrophobic Interaction, and Size Exclusion Chromatography — we develop tailored, multi-step purification protocols that deliver exceptional purity while maximizing recovery, often exceeding industry standards.
Key Capabilities
- Affinity chromatography (Protein A/G, His-tag)
- Ion Exchange & Hydrophobic Interaction
- Size Exclusion Chromatography (SEC)
- Multi-step protocol design
- Yield maximization & impurity removal
Protein Refolding Mastery
Rescuing functional protein from inclusion bodies
For challenging proteins expressed in inclusion bodies, our scientists possess deep expertise in optimizing protein refolding conditions. We employ proprietary methodologies to rescue functionally active protein from insoluble aggregates, significantly increasing product yield and viability.
Key Capabilities
- Inclusion body solubilization
- Refolding buffer & condition screening
- Proprietary refolding methodologies
- Activity verification post-refolding
- Scale-up from bench to pilot
Protein Formulation
Rational development for long-term stability & patient delivery
We ensure the long-term viability of your therapeutic through rational formulation development. This involves extensive excipient screening, stress testing (thermal, chemical), and buffer optimization to identify the optimal liquid or frozen dosage form. Our goal is to maximize shelf-life, maintain functional activity, and ensure patient-friendly delivery, mitigating aggregation and degradation risks for successful clinical trials.
Key Capabilities
- Excipient screening & optimization
- Thermal & chemical stress testing
- Liquid and frozen formulations
- Aggregation & degradation profiling
- Short and long term shelf stability studies
Engineering & Mutagenesis
Enhancing therapeutic performance and manufacturability
Enhance your therapeutic's performance and manufacturability. Our capabilities in site-directed mutagenesis and protein engineering are utilized to improve stability, increase activity, optimize half-life, reduce immunogenicity, or facilitate purification — giving your candidate a competitive edge.
Key Capabilities
- Site-directed mutagenesis
- Stability & half-life optimization
- Immunogenicity reduction strategies
- Purification tag engineering
- Fc-fusion & conjugation design
Validated Functional Assays
Critical potency & activity data for regulatory submissions
We develop and implement robust, qualified biological and binding assays (ELISA, SPR, cell-based assays) to measure product activity and potency, providing the critical data necessary to demonstrate efficacy and support regulatory submissions.
Key Capabilities
- Protein labeling for assays
- ELISA development & qualification
- Surface Plasmon Resonance (SPR)
- Cell-based potency assays
- Binding kinetics determination
State-of-the-Art Analytical Tools
Rigorous characterization at every development stage
Quality is ensured through rigorous characterization. We utilize a comprehensive suite of analytical technologies to confirm structural integrity, homogeneity, identity, and purity at every stage of development — providing the complete data trail required for regulatory confidence.
Key Capabilities
- SEC-HPLC, RP-HPLC and DSF
- Mass spectrometry (intact & peptide mapping)
- Absorbance & fluorescence spectroscopy
- SDS-PAGE, Western blot & cIEF
- Full characterization data packages